Kern, M., Bertram, B., & Milks, T. (2005). Metabolic response to ingestion of whey protein in active men. Medicine and Science in Sports and Exercise, 37(5), Supplement abstract 2191.

Males (N = 10) ingested 52 gm of whey protein isolate following an overnight fast. Blood was collected 60, 180, 360, and 480 minutes after consumption.

Leucine, glutamine, and insulin concentrations significantly peaked 60 minutes after consumption. Growth hormone peaked 180 minutes after consumption. Plasma testosterone, IGF-1, cortisol, and glucagons did not fluctuate significantly.

Implication. Metabolic responses that could favor anabolism are maximal relatively soon (1-3 hours) after ingestion of whey protein.

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